Because of its voltage dependence, the current activated by proctolin increases the amplitude of the oscillations generated by bursting neurons without producing a depolarization of the baseline (Figure 6A). The same effect is seen with muscarinic agonists such as pilocarpine or oxotremorine (Marder and Paupardin-Tritsch, 1978; Swensen and Marder, 2000). In contrast, nicotine, which activates a conventional nicotinic receptor (Marder and Eisen, 1984b; Marder and Paupardin-Tritsch,

1978), depolarizes Dasatinib nmr the baseline of the oscillator (Figure 6B) and can result in a depolarization block. Thus, the voltage dependence of the current elicited by proctolin and muscarinic agonists has a built-in brake that maintains the integrity of the burst generating mechanism in the pyloric pacemaker neurons (Marder and Meyrand, 1989). In addition to proctolin

and muscarinic agonists, a large number of other peptides including Crustacean Cardioactive Peptide (CCAP), RPCH, TNRNFLRFamide, SDRNFLRFamide, and Cancer borealis Tachykin-Related Peptide (CabTRP1a) activate the same voltage-dependent current (Swensen and Marder, 2000) and act on some of the same neurons (Figure 7A). Because these modulators converge onto the same current, they occlude each other’s actions (Figure 7B) (Swensen and Marder, 2000). Thus, if a neuron is already highly activated by one of these modulatory substances, a second of them will selleck be relatively ineffective. Modulators can enhance the amplitude of synaptic currents many-fold. For example, RPCH produces several-fold increases in the amplitude of the inhibitory LP to PD synapse in the pyloric network of the lobster Homarus americanus ( Thirumalai et al., 2006). Although this synapse is the major feedback to the pacemaker of the pyloric rhythm, this increase in synaptic strength does not necessarily change the frequency of the pyloric rhythm ( Thirumalai et al., 2006) because the effect of the inhibitory input

to an oscillator often saturates as synaptic strength is increased ( Prinz et al., 2003b). This saturation means that the network’s activity is de facto protected against Dichloromethane dehalogenase overmodulation of the feedback synapse to the oscillator. In motor systems central pattern generating networks drive muscles, and it is the muscle movement that is important for behavior. Brezina and colleagues (Brezina et al., 2005, 2000b; Brezina and Weiss, 2000; Zhurov and Brezina, 2006) have argued that coordinate modulation of muscles, neuromuscular junctions, and the central pattern generating circuitry ensures that the presynaptic activity generated in the motor neurons is appropriately matched to their muscle targets. This general principle, of correlated and coordinated modulation of multiple sites in a sensory-motor circuit is likely to be a general principle, found in many nervous systems (Taghert and Nitabach, 2012).