, 2012). Samples collected on May 26, 2011, revealed an abundance of Dolichospermum flos-aquae, Planktothricoides raciborskii, and Arthrospira sp., along with a minority population of M. aeruginosa, however M. aeruginosa was again the dominant species by the time samples were collected again in August. The only prolonged disruption of this trend was seen in 2012, where P. raciborskii was the most dominant species throughout the warm season spanning from July to

September. By 2013, large-scale blooms of M. aeruginosa were again observed, and lasted until the middle of November. MCs have been detected in the surface water since the beginning of our research in 2008 (Fig. 2). During the peak blooming periods between 2009 and 2013 (Fig. 2), MC concentrations frequently exceeded World Health Organization (WHO) guidelines for drinking water (1 μg/L; World Health Organization, 1999 and World BIBW2992 datasheet Health Organization, 2003). Concentrations remained high in years where M. aeruginosa was not the dominant species, however these concentrations were below the

1 μg/L limit, as the dominant species were either nontoxic (Arthrospira sp., 2008) or only weakly so (P. raciborskii, 2012). Seasonal changes were observed in the MC content of the surface sediment (0–1 cm depth; Fig. 3). MCs were readily detected in the reservoir sediment throughout the year, at concentrations Selleckchem AG-14699 approximately one order of magnitude higher than that of the surface water. These concentrations peaked in September 2009, with MC levels reaching 150 μg/kg water at station R2. MCs also persisted in deeper areas of the sediment. Residual levels were observed in the deep sediment collected by the KK-core sampler on November 19, 2008, June 11, 2009, and August 19, 2009, at station R2. MCs were detected as deep as 24–26 cm, which was the limit of the collection (see Fig. 4). Macrobenthos were collected over 11 different time points between April 2010 and August 2011. The majority of chilomonids were Microchironomus

tabarui, with a few Chironomus plumosus mixed in. The average wet weight of the macrobenthos during that period was 6.7 g/m2 at station R1 and 1.2 g/m2 for stations R2–R4 (see Table 1 and Table 2). Reservoir Cediranib (AZD2171) water is often discharged to maintain the water level at a depth of 1 m below mean sea level. On 16 September 2009, we received a sample of drainage water collected ∼40 min after the beginning of a discharge, which was collected by Mr. Ryoji Tokitsu, a local inhabitant (Movie 1). The MC concentration of this sample was 1.9 μg/L. According to the official data, 1.1 million tons of water were drained from the northern drainage gate on that day. Assuming that MC concentrations were constant throughout the drainage water, this amounts to ∼2.0 kg MCs exhausted in the surrounding bay.