Analysis of mycorrhiza A modification of a common mycological staining technique was used to clear and mark products. The ramification of the branches was also taken into account, the lengths of all the main branches rising from the soil, along with the lengths of all of the medial side branches, were measured and examined. Great roots were tested, while knotweed Capecitabine Antimetabolites inhibitor roots were hand separated in the melilot roots, and both were inspected and stained for the presence of mycorrhiza. The experiment was ended following the 2nd time in September 2007. At the end of the test, both the aboveground and below-ground biomass were measured, the fine roots were tested for mycorrhiza and larger roots and rhizomes were completely cleaned using air and water stress. They were then dried and ground for research. Melilot was allowed to grow without restriction during the primary season, but plants were over and over repeatedly cut during the next season to maintain a peak of 30 cm. Field experiment The middle of the 1 ha experimental low irrigated area are at a location of fifty 35 N, 13 52 E. That experiment field is a former spoil bank which was converted in to an arable field by organic manuring Meristem and ploughing and still shows a high clay content. In April 2006, 15-20 cm long rhizomes of pre developed Page1=46. bohemica were planted with a space of 100 70 cm and were immediately covered with dirt. Five plants were randomly sampled on each testing time in September and July of 2006, and in September, July and Might of 2007 and 2008. Flowers were dried above-ground and then washed and the below-ground biomass was measured. Since the samples from the pot experiment Si samples from each set were analysed for the same stilbenes and emodin. Normal studies The stilbenes ubiquitin ligase activity resveratrol, piceatannol and its glycosides, were analysed along side emodin in types of roots and knotweed rhizomes. Dry and finely ground samples were extracted with 60% ethanol, and the components were analysed using HPLC. Fig. 13 shows a normal record of the stilbenes and emodin tested by this technique. The soil samples were washed with water over a sieve. The roots were handseparated, cut in to 1 2 cm segments, cleaned with 10% KOH solution and stained with 0. 05% trypan blue in lactoglycerol. Origin portions were viewed under a microscope at 100 or 200 magnification and were screened for mycorrhizal colonisation. The presence or lack of AM colonisation was determined. The degree of mycorrhizal colonisation was examined using the grid line intersect strategy at 50 magnification under a dissecting microscope. The intensity and frequency of mycorrhizal colonisation were also calculated. Data analysis The data were analysed using SPSS 15. 0 statistical pc software. Normality of the data was examined and non normally distributed data were transformed by position.