037) and pathological grade (P = 0021)

037) and pathological grade (P = 0.021). RAD001 manufacturer Moreover, the overall survival of patients with negative IGFBP7 expression was significantly (P = 0.003) poorer than that of IGFBP7-positive patients. Cox regression analyses showed that IGFBP7 expression was an independent

predictor of overall survival (P = 0.02). Conclusion: The expression of IGFBP7 is significantly reduced in gastric cancer, which is associated with higher T stage and differentiation grade. IGFBP7 may serve as a prognostic marker as well as a potential therapeutic target for gastric cancer. Key Word(s): 1. gastric neoplasm; 2. RT PCR; 3. Western blotting; 4. survival analysis; Presenting Author: WEIHAO SUN Additional Authors: XIAOLIN LI, HAO ZHANG, KUN SUN, KAI ZHANG Corresponding Author: WEIHAO SUN Affiliations: The First Affiliated Hospital of Nanjing Medical University Objective: The current study evaluated the antitumor effects of Harmine (HM) on human gastric cancer both in vitro and in vivo. Methods: Growth inhibitory activity was assayed by the Methyl thiazolyl Smoothened Agonist concentration tetrazolium (MTT) assay, apoptotic staining and Flow cytometry analysis. The wound-healing and transwell invasion assays were performed to evaluate the effect of HM on inhibiting tumor invasion and metastasis. The protein expressions involved in regulating apoptosis

and invasion and metastasis were detected by western blot. Results: HM inhibited the proliferation of human gastric cancer cell lines BGC-823 and SGC-7901 in a dose- and time-dependent manner. In addition, HM effectively promoted the apoptosis of gastric cancer cells (Fig. 1) through dose-dependently inhibiting the expression of cyclooxygenase-2 (COX-2) (Fig. 2). Moreover, HM could induce apoptosis through a direct impact on many apoptosis-related proteins, such as Bcl-2 and Bax (Fig. 2). Most importantly, HM dramatically inhibited migration and invasion of gastric cancer by down-regulating the matrix metalloproteinase-2 (MMP-2) expression regulated by COX-2. In vivo, HM suppressed gastric xenograft tumor growth significantly. Fig. 1 Apoptosis

of BGC-823 and SGC-7901 cells detected by Flow cytometry. BGC-823 and SGC-7901 cells were treated with harmine at 0, 4, 8, and 16 μg/ml 上海皓元 for 24 hours. (A, B) Representative annexin V-FITC/PI stained BGC-823 (A) and SGC-7901 (B) cells. (C, D) The histograms on the right represent the rates of apoptotic cells in BGC-823 and SGC-7901 groups. All data represent the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 vs. control (0 μg/ml). Fig. 2 Effects of harmine on the COX-2, Bcl-2 and Bax protein expressions in BGC-823 and SGC-7901 cells. BGC-823 and SGC-7901 cells were treated with harmine at 0, 4, 8, and 16 μg/ml for 24 hours. (A, B) Representative COX-2, Bcl-2 and Bax protein expressions in BGC-823 (A) and SGC-7901 (B) cells detected by western blot analysis.

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