However, we could not detect any gross changes in the stromal immune cell component CH5424802 price or blood vessel density of fascin knockout tumors, and we recently reported that fascin loss is dispensable for growth of transplanted tumors.38 Fascin has been implicated in migration and invasion in vitro,

so it was surprising that fascin loss had no effect on invasion in vivo. We previously observed that only melanoma cell lines displaying elongated mesenchymal mechanisms of invasion were dependent on fascin.14 Collective invasion into bowel or peritoneal wall is not limited by loss of fascin and might also not be limited by matrix remodeling or invadopodia formation. Collective PDAC invasion could occur in physiological clefts between tightly packed collagen bundles or muscle strands,39 and fascin-mediated protrusions might not be crucial. We show that fascin null cells are less able to colonize the mesentery. Rho-associated colied-coil-containing protein kinase and myosin-mediated contractility are required for transmesothelial migration of human multiple myeloma and ovarian cancer cells.40 and 41 Rapamycin solubility dmso We

provide mechanistic evidence that fascin drives long filopodia that cross between the mesothelial cells and make initial contact with the substratum to aid transmigration. Our study suggests that, at least for PDAC, it is not invasion of the primary tumor, but rather colonization of the new site that is most affected

by fascin loss. The authors thank Joel Habener and Violeta Stanojevic of the Mass General Hospital, Boston, MA for their generous gift of slug antiserum. We also thank Colin Nixon of Beatson Histology Services, Matthew Neilson of Beatson Bioinformatics, and all staff of Biological Services Unit and the Beatson Advanced Imaging Resource imaging facility. Ang Li’s current affiliation is Laboratory of Mammalian Acetophenone Cell Biology and Development, The Rockefeller University, New York, NY. “
“Event Date and Venue Details from 2012 NORTHEASTERN WEED SCIENCE SOCIETY ANNUAL MEETING 03-06 JanuaryPhiladelphia, PA, USA Info: http://tinyurl.com/3rfqmnv. INTERNATIONAL ADVANCES IN PESTICIDE APPLI-CATION, WAGENINGEN, THE NETHERLANDS 10-12 January Info: www.aab.org.uk. [email protected]. 3rd GLOBAL CONFERENCE ON PLANT PATHOLOGY FOR FOOD SECURITY AT THE MAHARANA PRATAP UNIVERSITY OF AGRICULTURE AND TECHNOLOGY 10–13 Jan 2012 Udaipur, INDIA Voice: 0294-2470980, +919928369280 E-mail: [email protected] SOUTHERN WEED SCIENCE SOCIETY (U.S.) ANNUAL MEETING 23–25 January Charleston, SC, USA SWSS, 205 W. Boutz, Bldg. 4, Ste. 5, Las Cruces, NM 88005, USA Voice: 1-575-527-1888 E-mail: [email protected] Web: www.swss.ws 1st INTERNATIONAL WORKSHOP ON BAC-TERIAL DISEASES OF STONE FRUITS AND NUTS 14–17 FebruaryZurich, SWITZERLAND B. Duffy, Agroscope FAW, Schloss, Postfach 185, 8820 Waedenswil, SWITZERLANDE-mail: [email protected].