it can trigger dendritic and mononuclear cells to stop the apoptosis of virus-infected cells and start the anti viral defense. Liver fibrosis and its end stage cirrhosis represent the last common pathways of almost all chronic liver diseases. In these days, for that functions of Treg and Th17 cells during HBV disease Everolimus RAD001 and their implicit developmental links, the significance of Treg/Th17 balance continues to be often discussed in CHB pathogenesis. Under inflammatory situations, the growth facets and cytokines in microenvironments might trigger mitogen activated protein kinase pathways in T-cells. To date, three distinctive MAPK pathways have already been identified in mammalian cells: the extracellular signal regulated kinase pathway, the d Jun amino terminal kinase pathway and the p38 MAPK pathway. Recent studies have also implicated that phosphatidylinositide 3 kinase /AKT pathway activation is just a fundamental requirement for cell cycle progression and T cell growth. PI3K/AKT and mapk/erk pathways are crucial in shifting extracellular concept via membrane destined ligands, transmembrane receptors and cytoplasmic Mitochondrion extra messengers in to cell nucleus, thus controlling the gene expression that controls several important cellular functions including cell growth and apoptosis are important targets for antifibrogenic remedies. Glycyrrhizin, the principal active constituent of licorice root, is usually used in Asia to take care of patients with chronic hepatitis. GL could promote cell proliferation of hepatocytes therefore it could be used to help liver regeneration after liver injury. Besides, GL have anti viral, anti tumor and immunomodulatory capabilities. In patients with chronic hepatitis C, intravenous administration of GL can minimize lcd aminotransaminase degrees, therefore stopping liver fibrosis progression in addition to future hepatocellular carcinoma. Our previouswork had demonstrated CHK1 inhibitor that GL can reduce hepatotoxin induced liver damage and cirrhosis via inhibition of NF kappaB binding task, cell proliferation and collagen production of HSCs. However, the activity of GL has not been fully elucidated in liver fibrosis. Here this study was made both in vitro and in vivo to investigate whether GL can control CD4 T cell response via PI3K/AKT, ERK and JNK transmission pathways in mouse liver fibrosis designs. 6 to 8 week-old female BALB/c mice weighing 18?20 g were obtained from the Shanghai Laboratory Animal Centre and managed under specific pathogen free conditions with a 12 hour light/dark period and allowed free access to water and food. Concanavalin An and glycyrrhizin were obtained from Sigma Chemical Co. and dissolved in pyrogen free phosphate buffered saline and saline, respectively.