Methods: A total of 56 patients, in whom the initial standard triple therapy had failed to eradicate H. pylori infection, were randomly assigned into two groups. The first group (n = 28) received pantoprazole 40 mg twice daily, moxifloxacine 400 mg once daily and amoxicilline 1000 mg twice daily for 10 days. The second group received pantoprazole 40 mg twice daily, amoxicilline 1000 mg twice daily for 5 days followed by pantoprazole 40 mg twice daily, moxifloxacine 400 mg once daily and metronidazole 400 mg twice daily for the next 5 days. Testing for H. pylori

infection after treatment was done find more using the (13) C-urea breath test six weeks after completing the treatment. Results: 50 patients (89%) completed the study. The eradication rates were 71,4% (20/28) and 73% (19/26) in the first group and 75% (21/28) and 77% (17/22) in the second group by intention-to-treat (p = 0,04) and per-protocol (p = 0,08) analyses respectively. Compliance was higher in the second group. Adverse effects were described in 3 patients in the first group and in 5 patients in the second, but were mild and did not require discontinuation of

therapy. Conclusion: Considering better compliance and higher eradication rates, moxifloxacine BYL719 based sequential therapy represents favorable second line alternative for H. pylori infection. Key Word(s): 1. Helicobacter pylori; 2. second line; 3. sequential therapy; 4. triple therapy; Presenting Author: XUAN HUANG Additional Authors: BIN LV, SHUO ZHANG, QUN DAI, BING-BING CHEN, LI-NA MENG Corresponding Author: BIN LV Affiliations: the First Affiliated Hospital, Zhejiang Chinese Medical University Objective: Radix curcumae (RC)-derived diterpenoid C is recemtly obtained from RC ether extract by us, and its chemical properties and constitution are different

from curcumin and β-elemene. Our previous experiments have shown that RC-derived diterpenoid C has better anti-tumor activity and RC-derived diterpenoid C of high concentration can induce apoptosis. But it inhibit inflammation effect and mechanism is unclear. Methods: We used I-type Hp to infect human gastric epithelial GES-1 cell lines, and then Hp-infected GES-1 cells were medchemexpress treated with RC-derived diterpenoid C of different concentrations (5 ug/ml, 10 ug/ml, 20 ug/ml)and amoxicillin. The expression of P65, IKKα and IKKγ proteins was detected with Western blot, and the expression of interleukin (IL)-8, IL-6 and IL-4 was determined with ELISA method. Results: MTT indicated that the IC5 of RC-derived diterpenoid C and amoxicillin all were 5 ug/ml for gastric GES-1 cells. The expression of IL-8 was significantly increased, especially at 12 hour time point; and the expression of IL-4 was decreased in Hp-infected GES-1 cells. After Hp-infected GES-1 cells were treated with RC-derived diterpenoid C of different concentrations and amoxicillin, the expression of IL-8 was decreased at 12 h, 24 h, 48 h, 72 h points (P < 0.