Osthole, 7-methoxy-8-(3-methyl-2-butenyl)coumarin(Figure 1), is an active constituent of Cnidium monnieri (L.) Cusson, has been extracted from many medicinal plants such as Cnidium monnieri and other plants. Osthole has long been used in traditional Chinese medicine for the treatment of eczema, cutaneous pruritus, trichomonas vaginalis infection, and sexual dysfunction. Recent studies have revealed that Osthole may have antiproliferative[7], vasorelaxant[8], anti-inflammatory[9], antimicrobacterial[10],

learn more antiallergic[11], and preventing prophylactic effects in hepatitis[12]. Furthermore, the anticancer effect of Osthole has been reported in few papers[13–17]. These studies have revealed that Osthole inhibited the growth, invasion and metastasis of cancer cells. However, the effects of Osthole on human lung cancer cells remain unclear. Figure 1 The structure of Osthole. The PI3K/Akt signaling pathway is a critical transduction pathway which plays an important role in regulating cell proliferation, cell cycle and apoptosis[18]. Various types of cancer, including lung cancer, were reported to aberrantly check details activate this pathway[19]. Recent studies have shown that some anticancer-drugs could induce G2/M arrest accompanying the down-regulation of Akt[20, 21]. And the PI3K/Akt pathway participates in the regulation of Bcl-2 family proteins,

which are key regulators of the apoptotic pathway[22]. In the present study, we observed that Osthole induces G2/M arrest and apoptosis in lung cancer Cediranib (AZD2171) A549 cells. Osthole-induced G2/M arrest and apoptosis were associated with inhibition of the Cyclin B1, p-Cdc2 and p-Akt expressions, and up-regulation of the ratio of Bax/Bcl-2 proteins. Methods Reagents RPMI-1640, trypsin, penicillin and streptomycin were purchased from Biological Industries (Kibutz Beit Haemek, Ralimetinib solubility dmso Israel). Fetal bovine serum (FBS) was purchased from Solarbio Science&Technology (Beijing, China). 3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), Propidium iodide (PI), and Hoechst 33342 were purchased

from Sigma-Aldrich (St. Louis, USA). Annexin V-FITC and PI double staining kit were purchased from Key Gene (Nanjing, China). Osthole was purchased from the National Institute for the control of Pharmaceutical and biological products (Beijing, China), a 50 mM stock solution of Osthole was dissolved in DMSO and stored at -20°C. Antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). All other reagents were procured locally. Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented with 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin at 37°C in a humidified atmosphere of 5% CO2.