sellekchem A549 cells per se exhibited high migration potential which was reduced by 70% in A549 Spr, while A549 Inhibitors,Modulators,Libraries Env cells were unable to migrate. Similarly, BEAS 2B cells had good migration capability whereas BEAS 2B Env cells exhibited 80% reduction in their migration potential. The effect of Env transformation on the migration ability was drastic in A549 Env cells abrogating their migration potential, whereas in BEAS 2B Env cells, the effect was severe resulting in reduced migration ability. Therefore, we hypothesize that Sprouty2 might have a hand in the compromised migration potential of these cells. To verify the role of Sprouty2 in the regulation of cell migration, A549, A549 Env, BEAS 2B and BEAS 2B Env cells were treated with 200 pmoles of siRNA for Spro uty2 or with control siRNA and then allowed to migrate.

It was observed that siRNA mediated inhibition of Sprouty2 expression resulted in a corresponding enhancement in cell motility. Inhibitors,Modulators,Libraries The enhance ment was more prominent in A549 Env and BEAS 2B Env cells that had higher levels of Sprouty2 initially and consequently very low migration potential. Inhibition of Sprouty2 expression in the cells enhanced their migra tion ability, thereby confirming that Sprouty2 played an inhibitory Inhibitors,Modulators,Libraries role in cell migration. To investigate in detail the physiological consequences brought about by Env and Sprouty2, further investiga tions were carried out. Env induces proliferation and colony formation in A549 Env Proliferation and invasion are two distinct fundamental components occupying opposing ends of a spectrum in malignant cells and are not necessarily displayed by the same cells.

Invasion, migration and branching morpho genesis are exclusive characteristics of highly invasive cells while highly proliferative cells are highly tumori genic and display anchorage independent growth in soft Inhibitors,Modulators,Libraries agar. Anchorage independent growth is an attribute of oncogenic transformation by Env that causes cells to loose contact inhibition resulting in the formation of distinct foci in culture. The cell lines were further investigated for their proliferation potential and ancho rage independent growth. A549 Env had a higher proliferation rate with 4 fold more cells after 96 hours than A549 and A549 Spr. increased proliferation being a characteristic feature of oncogene induced transformation. On the other hand, both BEAS 2B and BEAS 2B Env had com parable Inhibitors,Modulators,Libraries proliferation rates.

Our results clearly demonstrate that the loss of invasive ability induced by JSRV Env is distinct from the enhanced proliferation function. Env mediated transformation had converted the highly invasive A549 cells into highly proliferative A549 Env cells. Our results suggest that the choice of invasion versus proliferation and tumor formation func tions is more likely to be governed by distinct check this pathways of signaling, which are probably evoked independently.