The anaerobic test was a modified form of the Wingate test [20]. The load on the ergometer platters, which was optimum for each athlete, was determined during a pilot study and amounted to 8.3% of BM on average, i.e. by 0.8 higher
than in the original. With this braking force, the athletes generated the greatest peak power. It consisted in pedaling for 30 seconds with maximal intensity using a mechanical bicycle ergometer Ergomedic 874E Crenigacestat datasheet manufactured by Monark. During the exercise, a computer recorded relative peak power (RPP) and relative total work (RTW), time to obtain peak power (toPP), time to maintain peak power (tuPP) and the fatigue index (FI). Graded test until fatigue A graded exercise test on a mechanical treadmill was carried out on the second day of the experiment, under similar ambient conditions.
After the determination of pre-exercise circulatory and respiratory indices, the subjects performed a 3-minute warm-up this website at the running speed of 2.3 m.s-1, and then the speed was increased by 0.5 m.s-1 ATM Kinase Inhibitor every three minutes. During the last 30 seconds of each loading segment, the subjects were taken blood samples from the earlobe in order to determine the lactate concentration in blood serum. The graded exercise was continued by the subjects until a subjective sensation of exhaustion. Using the apparatus of 919E type manufactured by Medikro, the indices of respiratory exchange were measured during the exercise every 30 seconds: tidal volume (TV), respiratory rate (F), minute ventilation (VE), minute oxygen uptake (VO2). Heart rate monitor VantageTM manufactured by Polar Electro was used for the measurements of heart rate (HR). Total time of exercise (t) and the distance (D) were also recorded. It was established based on a pilot study that the capillary blood samples used for Tau-protein kinase the determination of biochemical and morphological indices would be also taken from the earlobe three minutes after the exercise. This was the point when the highest lactate (La) concentration was found. All the exercise tests were performed in
an air-conditioned room in the Department of Physiology and Biochemistry of the Institute of Human Physiology. The project was approved by the Bioethical Committee at the Regional Medical Chamber (No. 76KBL/OIL/2008 of 17 September 2008). Special Judo Fitness Test Special Judo Fitness Test (SJFT) invented by one of the authors of the present study is an acknowledged tool of training control, implemented in many countries [11]. Visualized presentation was prepared at the University of Bath by Lance Wicks [21]. The test positively passed the statistic procedures determining the reliability and accuracy, and had normative data [11]. SJFT is a recognized tool used also in judo-related disciplines, such as ju-jitsu, hapkido etc. Statistical analysis The following descriptive statistics were calculated: mean, SD, median. Non-parametric methods were used, because not all parameters show normal distribution.