The purpose of this study Oligomycin A IC50 was to develop simple, fast, economical and validated analytical methods to quantify repaglinide in tablets using HPLC and UV spectrophotometry. Validation of the developed methods was done as per International Conference on Harmonization (ICH) guidelines.[11] The results obtained by these methods were statistically compared using analysis of variance. In addition the reliability and feasibility of these methods were evaluated, focusing on rountine quality control analysis. MATERIALS AND METHODS Materials Repaglinide, reference standard was obtained as a generous gift sample from USV Lab. Pvt. Ltd., Mumbai, India. Eurepa tablets labelled to contain repaglinide 2 mg, manufactured by M/S Torrent Pharmaceutical Ltd., Baddi (H.P.), India, were purchased from local market.

All the chemicals used were of AR and HPLC grade, obtained from E. Merck, India. Instrumentation and optimization conditions UV spectrophotometric analyses were carried out on a Shimadzu 1700 Double beam UV-Vis spectrophotometer, with 1.0-cm quartz cells. The wavelength of 241 nm was selected for the quantitation of repaglinide and the measurements were obtained against methanol as a blank. The HPLC analysis were carried out on Agilent 1120 Compact LC system composed of binary pump, manual injector, UV detector and Ezchrome EliteCompact software. The column used was Agilent TC-C18 (250 mm �� 4.6 mm i.d., 5 ��m particle size) and the mobile phase consisted of methanol and water (80:20 v/v, pH adjusted to 3.5 with orthophosphoric acid) at a flow rate of 1.0 ml/min.

Detection was performed on at 241 nm. Preparation of standard solution The standard stock solution of repaglinide 1000 ��g/ ml was prepared in methanol. For spectrophotometric method, further dilutions of aliquots of standard stock solution were carried out with methanol to reach the concentration range 5-30 ��g/ml for repaglinide. The absorbance of series of solutions was measured at 241 nm and found to be proportional to the corresponding concentrations of repaglinide. For HPLC method, the standard solutions were prepared by dilution of aliquots of the standard stock solution with mobile phase to reach the linearity range of 5-50 ��g/ml of repaglinide. Twenty microlitre of the each standard solution was injected to HPLC system. The peak areas were plotted against the corresponding concentrations to obtain the calibration graph.

Preparation of sample solution To determine the content of repaglinide in conventional tablets (label claim: 2 mg repaglinide per tablet), 20 tablets were weighed, their mean weight determined and finely powdered. A portion of tablet powder equivalent to 10 mg of repaglinide was accurately Dacomitinib weighed and dissolved in 30 ml methanol in 100 ml volumetric flask. The contents of the flask were sonicated for 15 min to dissolve repaglinide, volume was made up to the mark with same diluent and the resulting mixture was filtered.