Thirty-nine additional blind-coded Bartonella strains were correctly identified at the species level,
including 36 with a significant score. Altogether, these data demonstrate that MS is an accurate and reproducible tool for rapid learn more and inexpensive identification of Bartonella species.”
“We have studied the dynamic scanning of liquid-crystalline (LC) poly(p-phenyleneterephthalamide) sulfuric acid (PPTA-H(2)SO(4)) solution, and its blend with single-walled carbon nanotubes (SWNTs), by using a flat plate rotational rheometer. The effects of weight concentration and molecular weight of PPTA, as well as operating temperature, on dynamic viscoelasticity of the PPTA-H(2)SO(4) LC solution system are discussed. The transition from a biphasic system to a single-phase LC occurs in the weight concentration range of SWNTs from 0.1% to 0.2%, in which complex viscosity reaches the maximum at 0.2 wt% and the minimum MDV3100 order at 0.1 wt%, respectively, of SWNTs. With increasing SWNT weight concentration, the endothermic peak temperature increases from 73.6 to 79.9 degrees C. The PPTA/SWNT/H(2)SO(4) solution is in its plateau zone and storage modulus (G’) is a dominant factor within the frequency (omega) range of 0.1-10 rad/s. As omega increases, the G’ rises slightly, in direct proportion to the omega. The loss modulus (G ”) does not rise as a function of omega when omega < 1 s(-1), then when omega > 1 s(-1) G ” increases faster than
G’, yet not in any proportion to the omega.”
“Background: Dengue fever is the most important vector-borne viral disease. Four serotypes of dengue virus, DENV1 to DENV4, coexist. Infection by one serotype elicits long-lasting immunity to that serotype but not the other three. Subsequent infection by a different serotype is a risk factor for severe dengue. Domain III (ED3) of the viral envelope protein interacts with
cell receptors and contains epitopes recognized by neutralizing antibodies. We determined the serotype specificity and cross-reactivity of human IgMs directed against ED3 by using a well-characterized collection of 90 DENV-infected and 89 DENV-uninfected human serums.\n\nMethods: The recognitions between the four serotypes of ED3 and the serums were assayed with an IgM antibody-capture ELISA (MAC-ELISA) and artificial homodimeric antigens. The results were analyzed with selleck compound Receiving Operator Characteristic (ROC) curves.\n\nResults: The DENV-infected serums contained IgMs that reacted with one or several ED3 serotypes. The discrimination by ED3 between serums infected by the homotypic DENV and uninfected serums varied with the serotype in the decreasing order DENV1 > DENV2 > DENV3 > DENV4. The ED3 domain of DENV1 gave the highest discrimination between DENV-infected and DENV-uninfected serums, whatever the infecting serotype, and thus behaved like a universal ED3 domain for the detection of IgMs against DENV.