This [(3)H]-NPD1/PD1 also displayed specific and selective high affinity binding with isolated human neutrophils (K(d) similar to 25nM). Neither resolvin E1 nor lipoxin A(4) competed for [(3)H]-NPD1/PD1 specific binding with human neutrophils. Together, these results provide evidence for stereoselective specific binding of NPD1/PD1
with retinal pigment epithelial cells as well as human neutrophils. Moreover, they suggest specific receptors for this novel mediator in both the immune and visual systems. (C) 2009 Elsevier Ltd. All rights reserved.”
“Animal models that mimic human diabetic nephropathy are useful to identify key factors in pathogenesis of this disease, as well as the development of new therapies. Several mouse TPX-0005 cell line models of diabetes have features of human diabetic nephropathy, yet none of these completely fulfill the Animal Models of Diabetes Complications Consortium criteria and completely reproduce pathological and functional features of the human disease. The Akita mouse carries a mutation in the insulin-2 gene and, to date, only survives as heterozygotes that develop spontaneous type 1 diabetes. Here we show that Akita mice with mutation of both insulin-2 alleles (Akita knockout (KO)) survive if crossed onto
the Balb/c background. These mice develop hyperglycemia, more severe albuminuria, and mesangial sclerosis compared with heterozygous mice on the same genetic background. Interestingly, crossing these AkitaKO mice with integrin alpha
1KO mice, a check details model of exacerbated glomerulosclerosis after injury and also on the Balb/c background, resulted in a 16-fold increase in albuminuria, significant mesangial matrix expansion, nodular and diffuse glomerulosclerosis, and a 2-fold increase in glomerular basement membrane thickening when Sirolimus order compared with nondiabetic mice. Moreover, a significant decline in glomerular filtration was evident in the alpha 1KOAkitaKO mice at 6 months of age. Thus, the integrin a1KOAkitaKO Balb/c mouse represents a promising model presenting with most features of human diabetic nephropathy. Kidney International (2012) 81, 1086-1097; doi: 10.1038/ki.2011.474; published online 1 February 2012″
“The pontine micturition center, or Barrington’s nucleus, is an essential component in the micturition reflex. The purpose of the present study is to examine the connections between Barrington’s nucleus and sacral preganglionic neurons with an electrophysiological method using an intracellular recording technique. When the bladder pressure was near 0 mmH(2)O, electrical stimulation of Barrington’s nucleus either evoked no postsynaptic potential or evoked very small excitatory postsynaptic potentials (EPSPs) with ambiguous onset, in sacral preganglionic neurons. However, when the bladder showed micturition contraction, electrical stimulation of the nucleus evoked clear EPSPs in sacral preganglionic neurons. Latencies of EPSPs ranged from 21.9 to 47.5 ms.