Western blotting The antibodies used in the Western blot, following manu facturers protocols, were anti DLC1, anti PAI one and anti B actin. Tissue lysates containing equal amounts of complete protein have been separated by SDS Page. To detect proteins of interest, enhanced chemilumines cence technique was used in accordance to the suppliers proto col. Relative ranges of proteins have been estimated densitometric ally making use of B actin as inner reference. Statistical analysis SPSS 17. 0 software was employed for the statistical evaluation. Continuous variables had been expressed as X s. Chi square check, Logistic regression evaluation and Partial Correlate had been carried out to evaluate the association among DLC1 and PAI 1 with clinicopathological traits. Overall sur vival was estimated by Kaplan Meier curves and multi variate Cox examination. The relationships amongst DLC1 and PAI one protein expression were analyzed by Pearsons cor relation coefficient.
Final results have been thought of statistically important when P less than 0. 05. Outcomes Expression of DLC1 and PAI 1 in epithelial in the know ovarian cancer tissues and normal ovarian tissues Good staining for DLC1 observed in malignant and ordinary ovarian tissues were 33 75 and 25 25 respectively, but were 51 75 and 9 25 for PAI one. The Western Blotting showed that the expression of DLC1 protein in normal and malignant ovarian tissues had been and,but and for PAI 1. The expression of DLC1 in ovarian carcinoma tissues was considerably lower than that in standard ovarian tissues,whereas it was converse for PAI one. Association of DLC1 and PAI one expression using the clinicopathologic characteristics of ovarian cancer As shown in Table 1, the expression of DLC1 and PAI 1 had been substantially associated with FIGO stage and lymph node metastasis in ovarian carcinoma.
Additionally, DLC1 was also linked with ascites, and PAI 1 was re lated with histological differentiation. The correlation amongst DLC1 and PAI one read full article in epithelial ovarian carcinoma Between the 75 specimens of EOC, there were 15 favourable for DLC1 and damaging for PAI one, also as 33 damaging for DLC1 and good for PAI 1. This result suggests a damaging correlation among the expression of DLC1 and PAI one. Associations of DLC1 and PAI one expression with all the prognosis of ovarian cancer Partial Correlate examination showed the expression of DLC1 was negatively relevant with FIGO stage,asci tes,lymph node metastasis,but positively linked with prognosis. The ex pression of PAI 1 was positively associated with FIGO stage,histological differentiation,lymph node metastasis,but negatively re lated with prognosis.
Logistic Regression examination indicated the expression of DLC1 was closely relevant with FIGO stage,the expression of PAI one was closely connected with lymph node metastasis,and the expression of DLC1 mixed with PAI one had been important correlative factors with prognosis. Furthermore, Kaplan Meier survival curves demonstrated that ovarian cancer sufferers with damaging expression of DLC1 and optimistic expression of PAI one had the worst general survival time in comparison with other patients. Multivariate Cox analysis showed that only DLC1 combined with PAI one expression had been in dependent risk aspects of prognosis.