m. and 1 a.m. The diagnosis of filariasis was established by immunochromatographic rapid test (ICT Diagnosticx,

Binax NOW®) that uses polyclonal and specific monoclonal antibodies15 and by the polycarbonate membrane filtration technique. For the ICT card test we used 100 μL of blood on the specified region of the card, which was closed after JQ1 approximately 30 seconds. The card was read after 10 minutes. When the W. bancroft antigen is present in the sample, it is captured by the AD12 monoclonal antibody present in the nitrocellulose strip and depicted as a pink bar. The test was considered positive when two pink lines were identified, and negative when only the control line was displayed. 15 The search and quantification of circulating microfilariae were performed using the filtration technique with a 3 μm pore polycarbonate membrane. When the results with 1 mL blood were negative, further 9 mL were subsequently filtered for confirmation of negativity. The membrane was fixed and stained by Carazzi’s haematoxylin, and then read by optic microscopy (160x). The filtration technique was considered negative when no microfilaria was identified in 10 mL of blood, and positive in the presence of 1 ≥ microfilaria. For the investigation of intestinal parasites, three stool samples obtained on different days and kept in 10% formaldeid were analyzed using the Hoffmann, Pons and Janer method. The test was considered positive if one or more

parasites were found in any of the samples. Data entry and validation were done in the Epi Info database, version 6.04d, with double input and mTOR inhibitor correction of the identified differences. Analysis of the descriptive statistics (mean and distribution of selleck compound frequencies)

was done with Epi Info version 7. The study was approved by the Research Ethics Committee of Aggeu Magalhães Research Center/Fiocruz – PE (CAAE 0069.0 095 000-06). The tests results were notified to the children’s’ guardians by the schools, and all those with filarial infection or intestinal parasites were given the specific treatment. Tests for filarial and intestinal parasites were concomitantly performed for 159 children. Mean age was 9.8 years (5-18); 53.4% (85/159) were male and 46.6% (74/159) were female. Intestinal parasites were identified in 64.2% children (102/159), and filariasis was diagnosed in 13.8% by the ICT technique (22/159). Concurrent filarial and intestinal parasites were diagnosed in 9.4% (15/159) students, and 31.5% (50/159) were free from any parasites. From the total, 87 (54.7%) individuals were positive for intestinal parasites and negative for filariasis, and 7 (4.4%) were negative for intestinal parasites and positive for filariasis. Among the individuals who were positive for intestinal parasites, 45% (46/102) had more than one parasite identified in stools. Geohelminths occurred in 72.5% (74/102), with A. lumbricoides and T. trichiura being the most prevalent parasites.