These cells were subdivided into two populations: CD11bhiLy6Chi (classical) and CD11bhiLy6Clow (non-classical) monocytes (Fig. 2A). In the fetal pancreas two precursor populations were present with a similar phenotype as blood monocytes. Due to a genetic abnormality of the Ly6C gene in NOD mice the expression of Ly6C is present, but significantly lower than in control mice 16. The phenotype of the two monocyte populations was further characterized using Ab against CD11c, F4/80 and CD86. In blood, Ly6Chi monocytes were CD11clowF4/80+CD86low

in both C57BL/6 and NOD mice (Fig. 2B). Ly6Clow blood monocytes expressed CD11c. Two CD11c+ cell populations were observed: CD11clow and CD11chi. The Ly6Clow blood monocyte population of NOD mice

had more CD11chi cells than in C57BL/6 mice. Ly6Clow blood monocytes were F4/80+CD86low in both strains. In the fetal pancreas Ly6Chi cells were CD11c−F4/80+CD86− Ridaforolimus in vitro Nutlin-3a purchase in C57BL/6 and NOD mice. In the fetal pancreas Ly6Clow cells were F4/80+CD86− and expressed CD11c, although not that high as the Ly6Clow blood monocytes. No differences were observed between C57BL/6 and NOD fetal pancreas. Thus, in the fetal pancreas two myeloid precursor populations (Ly6Chi and Ly6Clow) were present. These cells showed a similar expression of F4/80 as blood monocytes, but had a lower CD11c expression on Ly6Clow cells and lacked CD86. To show that ER-MP58+ cells in the fetal pancreas are able to develop into

CD11c+ DCs, ER-MP58+ cells were isolated by cell sorting followed by culture with GM-CSF. After culture for 8 days the generated cells displayed a typical DC appearance with dendrites (Fig. 3A). More than 40% of these cells expressed CD11c and expressed MHCII and the co-stimulatory molecule CD86 (Fig. 3B). The absolute number of generated CD11c+ cells from cultured pancreatic ER-MP58+ cells was significantly higher in NOD than in C57BL/6 (Fig. 3C). The generated CD11c+ cells from NOD and C57BL/6 were able to quench DQ-OVA showing the capability to process Sulfite dehydrogenase antigens (Fig. 3D). No significant difference in the DQ-OVA expression was detected between NOD and C57BL/6. A property of precursors is their proliferative capacity; therefore the proliferation of precursors in the fetal pancreas was analyzed by flow cytometry using Ki-67. In NOD fetal pancreas the number of Ly6ChiKi-67+ cells was significantly higher than in C57BL/6 (2.5-fold). No difference was found in the number of Ly6ClowKi-67+ cells between NOD and C57BL/6 (data not shown). To determine the proliferative capacity of ER-MP58+ cells in culture we used CFSE labeling. ER-MP58+ cells from the fetal pancreas, fetal liver, adult BM and blood were labeled and cultured with GM-CSF. Microscopic evaluation on day 4 of the GM-CSF culture of ER-MP58+ cells from the NOD fetal pancreas revealed increased cell numbers compared to C57BL/6 and BALB/c cultures (Fig. 4A).