We would also like to clarify the figure legends of Figures 2, 4C–4E, S1B, S3A, and S3B. Western blots were developed using the Odyssey Imaging System (LI-COR Biosciences), which allows detection of multiple primary antibodies probed on the same blot. Thus, the beta-actin loading controls are the same for two different primary antibodies—for example, Figures 2A and 4C. “
“(Neuron 73, 713–728; February 23, 2012) The data sets in this paper have been now deposited and released in NCBI with the
GEO accession numbers GSE40431, GSE40506, and GSE40510. “
“The mammalian IGF2 mRNA-binding protein family (Gene symbol: IGF2BP) comprises three RNA-binding proteins with a conserved domain structure Wnt inhibitor including two N-terminal RNA recognition motifs (RRM) and four C-terminal hnRNP K homology (KH) domains (Fig. 1a; reviewed in: ). Diverse biological roles and distinct target mRNAs identified for the individual IGF2BP family members account for the numerous synonyms and aliases assigned to protein family (CRD-BP, KOC, ZBP, VICKZ or Vg1RBP/Vera ABT-888 purchase in Xenopus). The first family member described was IGF2BP1, which was initially identified as a protein involved in the stabilization of the MYC mRNA . The protein prevents MYC
mRNA degradation by binding to the coding region instability determinant (CRD) and thereby promotes tumor cell proliferation and survival in various cancer contexts (reviewed in: ). Later on, IGF2BP1 was found to control the subcellular sorting of the ACTB mRNA in primary fibroblasts and neurons by binding to the cis-acting zipcode in the ACTB mRNA’s 3′UTR . By controlling the spatially restricted translation of the ACTB mRNA, IGF2BP1 was proposed to enhance neurite outgrowth and axonal guidance (; reviewed
in: ). The human IGF2BP2 was first described in 1999 due to its association with the IGF2 mRNA . Later on the protein, Oxygenase also termed p62, was proposed as an auto-antigen in hepatocellular carcinoma . Most notably, however, single nucleotide polymorphisms (SNPs) have been identified in the second intron of the human IGF2BP2 gene. These were correlated with an elevated risk of type two diabetes by various studies (reviewed in: ). Consistently, IGF2BP was recently identified as a modulator of mTOR signaling and IGF2 mRNA translation . The human IGF2BP3, which of all human family members shows the highest similarity to Xenopus Vg1/RBP, was initially termed KOC and identified due to its high abundance in pancreatic cancer tissue . Since its first identification a bulk of literature reported IGF2BP3 to be the mainly expressed family member in human cancer (reviewed in: ). Despite their high degree of similarity the IGF2BP proteins exhibit quite different expression patterns (reviewed in: ).